منابع مشابه
Increased sensitivity of the microbiological assay for biotin by Lactobacillus plantarum.
Addition of Tween 80 to biotin assay medium containing acid-hydrolyzed casein as the amino acid source caused marked growth of Lactobacillus plantarum ATCC 8014 in the absence of added biotin. This growth-promoting activity could be eliminated by treating the "vitamin-free" Casamino Acids (Difco) with activated charcoal (Darco G-60) at pH 3.5 for 30 to 60 min. Incorporation of Tween 80 and char...
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Although vitamin B12 can be assayed biologically with mice, chicks or rats, the microbiological method of assay is preferred since it is more rapid and economical. One serious shortcoming of many microbiological B12 assay procedures is the lack of specificity when crude materials are assayed. Crude materials can contain inhibitory or stimulatory factors which cause serious errors in assay resul...
متن کاملA Microbiological Assay Method for Thiamine
It is generally recognized that the most useful and convenient means of quantitatively determining members of the vitamin B complex involve microbiological methods. Several methods of this type have been proposed for the assay of thiamine but each one in use today possesses certain inherent disadvantages that limit its value. The fermentation method of Schultz, Atkin, and Frey (1,2), based on t...
متن کاملRapid microbiological assay for chloramphenicol and tetracyclines.
An agar diffusion assay for chloramphenicol and tetracyclines is described, using inhibition of hemolysis by Clostridium perfringens as a biological indicator of antimicrobial activity. Well-defined zones formed after a 1.5- to 2-h incubation in an anaerobic chamber or after 3 h using a GasPak system. Serum levels of chloramphenicol as low as 2.0 mug/ml could be reproducibly determined with thi...
متن کاملMultipoint microbiological assay for detecting f-lactamase
A multipoint microbiological assay for determining f-lactamase production by clinical isolates of bacteria was evaluated. With strains of Haemophilus influenzae, Neisseria gonorrhoeae, and Branhamella catarrhalis there was excellent correlation between results obtained using this method and those obtained using the chromogenic cephalosporin reference method. The multipoint method is an inexpens...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1942
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)45089-2